Journal: Scientific Reports

Article Title: A novel variant p.Y250C of FZD4 influences Norrine/β-catenin signaling pathway that associates with familial exudative vitreoretinopathy (FEVR)

doi: 10.1038/s41598-025-34442-0

Figure Lengend Snippet: Detection of wild-type and mutant FZD4 on downstream signal proteins by luciferase reporter gene assay. FZD4-wild-type (FZD4-WT) and FZD4-mutant (FZD4-MU) represent the relative luciferase activity ascertained from the transfection of wild-type FZD4 plasmid and mutant FZD4 plasmid into Hela cells, respectively. Data are representative of three independent experiments. The experimental data are presented as the mean ± standard deviation (mean ± SD). Differences between groups were assessed using the Student’s t -test, and * P < 0.05.

Article Snippet: The human cervical cancer HeLa cell line was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and maintained at a temperature of 37 °C in Dulbecco’s Modified Eagle Medium (DMEM) enriched with 10% Fetal Bovine Serum (FBS) and 1% penicillin/streptomycin.

Techniques: Mutagenesis, Luciferase, Reporter Gene Assay, Activity Assay, Transfection, Plasmid Preparation, Standard Deviation

Journal: Scientific Reports

Article Title: A novel variant p.Y250C of FZD4 influences Norrine/β-catenin signaling pathway that associates with familial exudative vitreoretinopathy (FEVR)

doi: 10.1038/s41598-025-34442-0

Figure Lengend Snippet: Immunostaining for FZD4 and Calnexin. The immunostaining for wild-type or mutant FZD4 and Calnexin in Hela cells. FZD4 protein shows in the green image on the left side of the panel; Calnexin displays with red image; nucleus shows in the blue image; the merge of them shows on the right side. Scale bar: 10 μm. Magnification: × 50.

Article Snippet: The human cervical cancer HeLa cell line was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and maintained at a temperature of 37 °C in Dulbecco’s Modified Eagle Medium (DMEM) enriched with 10% Fetal Bovine Serum (FBS) and 1% penicillin/streptomycin.

Techniques: Immunostaining, Mutagenesis

Journal: Scientific Reports

Article Title: A novel variant p.Y250C of FZD4 influences Norrine/β-catenin signaling pathway that associates with familial exudative vitreoretinopathy (FEVR)

doi: 10.1038/s41598-025-34442-0

Figure Lengend Snippet: Subcellular localizations and nuclear entry ratios of wild-type and mutant FZD4 proteins. ( A – C ) Subcellular localization of wild-type FZD4 protein express throughout the cytoplasm in HeLa cells, without highlights. ( D – F ) Subcellular localization of wild-type FZD4 protein aggregate on cell membrane and cytoplasm of HeLa cells, with highlights. ( G – I ) Subcellular localization of mutant FZD4 protein express throughout the cytoplasm in HeLa cells, without highlights. ( J – L ) Subcellular localization of mutant FZD4 protein aggregate on nucleic membrane and nucleus of HeLa cells, with highlights. The highlights represent the aggregation of GFP-labeled FZD4 protein. FZD4 protein shows in the green image on the left side of the panel; nucleus shows in the blue image in the middle; the merge of them shows on the right side. M: The ratio of wild-type FZD4 and mutant FZD4 proteins entering the nucleus. FZD4-WT represents the proportion of wild-type FZD4 protein entering the nucleus, while FZD4-MUT denotes the proportion of mutant FZD4 protein entering the nucleus. * P < 0.05. Scale bar: 5 μm. Magnification: × 50.

Article Snippet: The human cervical cancer HeLa cell line was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and maintained at a temperature of 37 °C in Dulbecco’s Modified Eagle Medium (DMEM) enriched with 10% Fetal Bovine Serum (FBS) and 1% penicillin/streptomycin.

Techniques: Mutagenesis, Membrane, Labeling